Adipose Tissue-derived Microvascular Fragments as Vasculariz
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Transplantation of dental pulp stem cells (DPSCs) has emerged as a novel strategy for the regeneration of the lost dental pulp after pulpitis and trauma. This recent study suggests that Co-transplantation with Adipose tissue-derived microvascular fragments (ad-MVFs) promotes angiogenesis and revascularization leading to robust regeneration of dental pulp.

Adipose tissue-derived microvascular fragments (ad-MVFs) were isolated from human adipose tissues. Apoptosis and senescence of DPSCs cultured in conditioned media were evaluated to explore the effects of ad-MVFs on DPSCs. DPSCs combined with ad-MVFs were inserted into the human tooth root segments (hTRSs) and implanted subcutaneously into immunodeficient mice. Regenerated pulp-like tissues were analyzed by hematoxylin and eosin (H&E) and immunohistochemistry. The vessels in regenerated tissues were analyzed by Micro-CT and immunofluorescence.

--The isolated ad-MVFs contained endothelial cells and pericytes. Ad-MVFs effectively prevented the apoptosis and senescence of the transplanted DPSCs both in vivo and in vitro.

--Combined with DPSCs, ad-MVFs obviously facilitated the formation of vascular networks in the transplants.

--DPSCs combined with ad-MVFs formed dental pulp-like tissues with abundant cells and matrix after 4-week implantation.

--Supplementation of ad-MVFs led to more odontoblast-like cells and increased formation of mineralized substance around the root canal.

Conclusively, co-transplantation with ad-MVFs promotes the angiogenesis and revascularization of transplanted DPSCs aggregates, leading to robust regeneration of dental pulp.

Journal of Endodontics
Source: https://doi.org/10.1016/j.joen.2021.04.012
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