Evaluation of, Singlet oxygen formation during accelerated a
A Study was conducted to evaluate the singlet oxygen (1O2) production of oxygen assisted %0.1 riboflavin and ultraviolet-A (UVA) crosslinking therapy (with and without oxygen assistance), in combination with standard, accelerated and hyper-accelerated procedures via an important quantitive marker of 1O2 which is the photo-oxidation of 1,3 diphenylisobenzofuran (DPBF).

%0.1 riboflavin-containing wells were irradiated with UVA light (365-nm wavelength) with or without 2-4-6-8L/min oxygen flow assistance. Measurements of decrease in absorbance of DPBF were made in 30mW (hyper-accelerated), 9mW (accelerated), and 3mW UV-A (standard) applications, and with additional 2-4-6-8L/min oxygen flow in 30mW and 2L/min oxygen flow in 9mW. A total of 8 different UV-A irradiance with and without oxygen supplementation groups were formed.

--2L/min oxygen assisted accelerated UV-A irradiance group has shown a greater decrease in DPBF absorbance compared to Dresden protocol.

--Also, Dresden protocol has shown a greater decrease in DPBF compared to all groups except accelerated crosslinking with 2L/min oxygen.

--Oxygen assisted hyper-accelerated crosslinking groups were showed greater reduction in DPBF absorbance compared to standard crosslinking without oxygen groups.

In conclusion, oxygen supplementation may raise singlet oxygen generation to levels comparable to the Dresden Protocol in the accelerated group. Furthermore, increased singlet oxygen formation with oxygen supplementation versus regular UV-A application may be considered promising in terms of shortening the crosslinking therapy.

Source: https://www.nature.com/articles/s41433-021-01731-7?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed:+eye/rss/current+(Eye+-+Issue)