The high-risk types of human papillomavirus (HPV) are a major cause of invasive cervical cancer and cervical intraepithelial neoplasia. New methods have been developed for the detection of cervical cancer, including examining molecular HPV DNA detection in vaginal and cervical samples. Women with multiple sexual partners have a higher risk of cervical cancer. The study was determined to seek the difference of high-risk HPV DNA test results between women with multiple sexual partners versus single sexual partners. The study was an observational analytic study with a cross-sectional design. Participants were 20 women with multiple sexual partners in prostitution areas and 20 single sexual partner women. Sampling for HPV DNA test was done by medical doctors and midwives. High-risk HPV DNA tests were examined with hybrid capture technique at Prodia Laboratory. Results: Among 40 participants, 9 participants from multiple sexual partners, and 3 participants from a single sexual partner were positive of high-risk HPV DNA test. The results of the prevalence rate (PR) analysis showed a value of 3.00 with confidence interval of 95%. The study shows that having multiple sexual partners is a potential risk factor for cervical cancer. From this study, it can be concluded that there was significant difference in high-risk HPV DNA test in women with multiple sexual partners compared to women with a single sexual partner. Women with multiple sexual partners are at risk to get positive of the high-risk HPV DNA test for 3.00 times compared to a single sexual partner. Source: http://ispub.com/IJGO/23/2/54576.