Honey as a Storage Media : Evaluation of Periodontal Ligamen
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The International Association of Dental Traumatology identifies avulsion as a dental emergency accounting for 0.5–16% of all injuries to the teeth. Avulsion or exarticulation has been defined by the World Health Organization’s (WHO) adaptation of Andreasen’s Classification as “total displacement of a tooth out of its alveolar socket.” The most commonly affected teeth are the maxillary central incisors. Avulsion injuries most often tend to occur in children between 7 years and 9 years of age, at the time the permanent incisors are in an active stage of eruption. An ideal storage medium is one that is capable of maintaining PDL cell viability alongside presenting clonogenic capacity, antioxidant property, no or minimal microbial contamination, ready accessibility, and has a low cost. So far, Hank’s balanced salt solution(HBSS) has been considered the gold standard for transport of an
avulsed tooth.

Various other types of wet storage media have been
investigated, but practicalities of their use such as limited shelf life, high cost, and, most importantly, lack of availability at the site of the accident make most of them less ideal. The literature has shown honey to have broad-spectrum antibacterial properties, typically against microorganisms infecting contaminated wounds. It has potent wound-healing properties, a high antioxidant capacity is easily available, and has a long shelflife, fulfilling most of the ideal requirements of storage media. A study was conducted to evaluate the viability of human periodontal ligament (PDL) cells in honey when used as a storage media at different time intervals. Fifty freshly extracted human premolars were divided into four experimental groups: group I: stored in honey immediately after the extraction; group II: stored in honey after 30 minutes extraoral dry time; group III: in Hank’s balanced salt solution (HBSS) immediately (positive control); and group IV: bench-dried for 8 hours with no media (negative control). Groups I, II, and III were further divided into three subgroups, to test viability at different time intervals of 3, 6, and 24 hours.

The PDL tissue-derived was subjected to the trypan blue
dye exclusion test. The number of viable cells was estimated with a hemocytometer and the data were statistically analyzed. Groups I and III showed no statistically significant difference in the percentage of viable PDL cells after 3 hours and 6 hours, and group II had a statistically lower percentage of viable cells compared to groups I and III. After 24 hours, group III had the highest percentage of viable cells. Group IV had a consistently lower percentage of viable cells.

Within the limitations of this study, it appears that honey may be as efficient as HBSS for the storage of avulsed teeth for up to 6 hours. Commercial honey meets most requirements of an ideal storage media. Being more readily available, inexpensive, and having several therapeutic properties can make it a popular storage media for a short duration of storage.

Source: https://www.wjoud.com/doi/WJOUD/pdf/10.5005/jp-journals-10015-1756