Impact of Incubation Conditions on In Vitro Phosphatidylchol
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Polar lipids play an important role in the tear film's stabilization. The effect of incubation time, lipid concentration, and artificial tear solution (ATS) replenishment on the absorption of phosphatidylcholine (PC) onto traditional hydrogel (CH) and silicone hydrogel (SH) contact lens materials was investigated in this review.

4 SHs and 2 CH lens materials (n = 4) were soaked in a complex ATS containing radioactive 14C-PC as a probe molecule. Phosphatidylcholine uptake was monitored at various incubation time points (1, 3, 7, 14, and 28 days), with different ATS lipid concentrations (0.5×, 1×, 2×) and with and without regular replenishment of the ATS. Phosphatidylcholine was extracted from the lenses, processed, and counted by a beta counter, and accumulated PC was extrapolated from standard lipid calibration curves.

Results:
--All materials exhibited increasing PC deposition over time.

--Conventional hydrogel materials showed significantly lower PC uptake rates than any of the SH materials.

--Increasing lipid concentration in the ATS resulted in increased PC binding onto the contact lens materials.

--Replenishing the ATS every other day, however, impacted the PC deposition differently, showing increased binding on CHs and reduced PC deposition for SH materials.

Finally, the duration of incubation, lipid concentration in the ATS, and incubation solution renewal all affected the amount of PC that sorbed onto different lens materials and should be taken into account in future in vitro deposition studies.

Source: https://journals.lww.com/optvissci/Abstract/2021/04000/The_Impact_of_Incubation_Conditions_on_In_Vitro.5.aspx
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