Kisspeptin as a Marker for Hyperandrogenemia in Women with P
Now open: Certificate Course in Management of Covid-19 by Govt. Of Gujarat and PlexusMDKnow more...Now open: Certificate Course in Management of Covid-19 by Govt. Of Gujarat and PlexusMDKnow more...
The polycystic ovarian syndrome is related to a disturbance in the hypothalamic-pituitary-gonadal axis. Kisspeptin has been recognized for its important role in gonadotropin-releasing hormone secretion initiation at puberty, regulatory secretion of luteinizing hormone during the ovulation process, and its relation to polycystic ovarian syndrome was found to be implicated in the hypothalamus-pituitary-ovary axis disturbance observed in polycystic ovary syndrome.

A case-control study was performed; it involved 87 women divided into two groups: 44 women diagnosed with polycystic ovary syndrome, 22 women with BMI equal or more than?25?kg/m2 and 22 women with BMI?less than?25?kg/m2, and another 43 women without Polycystic ovarian syndrome, 22 women with BMI equal or more than?25?kg/m2 and 21 women with BMI?less than?25?kg/m2. Hormonal, metabolic profiles, and hirsutism scores, as well as serum kisspeptin level, were assessed by using Human Kisspeptin 1(KISS-1), ELISA Kit.

The blood samples between days 2 and 5 of the menstrual cycle were drawn by a disposable sterile syringe and collected in EDTA containing tubes (as an anticoagulant), and the hormonal profile was measured using a biotech ELISA reader. Serum level of kisspeptin was significantly higher in Polycystic ovarian syndrome compared to control.

The current study showed that kisspeptin was significantly higher in Polycystic ovarian syndrome compared to control (322.4 vs. 235.3?ng/L), and after performing ROC analysis, kisspeptin showed good ability to predict Polycystic ovarian syndrome, with serum level equal or more than 271.234?ng/L predicting Polycystic ovarian syndrome; at this point, kisspeptin showed higher specificity and lower sensitivity with 81.6% accuracy. In addition, the positive likelihood ratio (luteinizing hormone) was 6.55 which indicates that this test can increase the index of suspicion for confirming the Polycystic ovarian syndrome diagnosis by 35–40%, while its negative luteinizing hormone indicates it has 25–30% ability to exclude the diagnosis of Polycystic ovarian syndrome. In the current study, the effects of kisspeptin on the release of reproductive hormones were examined. All the above findings have identified kisspeptin as a possible diagnostic marker for Polycystic ovarian syndrome.

Source:https://www.hindawi.com/journals/ogi/2020/5216903/
Like
Comment
Share