Knockdown of Notch ligand Jagged1 reduces osteosarcoma proli
Osteosarcoma is the most common primary bone cancer occurring in young adults and the 5-year survival rate of patients with metastatic osteosarcoma is <30% due to high metastatic recurrence and drug resistance. Notch is a highly conserved cell to cell signaling pathway in evolution, and Jagged1 is an important ligand of Notch. Although some studies have found that Notch receptors and ligands including Jagged1 were highly expressed in osteosarcoma tissues and osteosarcoma cells, their role is still not clear.

Tumor tissues were obtained from 68 patients, and immunohistochemical staining was used to divide them into groups based on Jagged1 expression. The expression of Jagged1 was detected using real-time quantitative PCR and Western blotting. The expression of Jagged1 in F5M2 cells was knocked down using siRNA. The proliferation of F5M2 cells with and without Jagged1 knockdown was identified and analyzed using the colony formation assay and MTT. The migration and invasion of F5M2 cells were identified using a transwell assay.

Results:
--It was found that the high expression of Jagged1 is closely related to the metastasis and recurrence of osteosarcoma in 68 clinical specimens.

--The expression of Jagged1 in F5M2 cells with high metastasis was significantly higher than that in F4 cells with low metastasis.

--Knockdown of Jagged1 led to lower ability of proliferation, migration, and invasion in F5M2 cells.

Finally, increased Jagged1 expression has been related to osteosarcoma metastasis and recurrence. Jagged1 knockdown reduced osteosarcoma cell proliferation, migration, and invasion significantly. Inhibiting Jagged1 may be an effective treatment for metastatic osteosarcoma, according to the results.

Source: https://josr-online.biomedcentral.com/articles/10.1186/s13018-021-02372-y
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