MP-RNA (SAT) with MP-IgM (PA) detection methods used for Myc
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This retrospective study aimed to compare the diagnostic methods for Mycoplasma pneumoniae in children and evaluate their values. Aetiological diagnosis of pneumonia caused by Mycoplasma pneumoniae (M. pneumoniae, MP) is a constantly challenging issue.

830 children with community-acquired pneumonia were selected. On the first day of hospitalization, sputum, throat swab and venous blood samples were collected to analyse MP-IgM (particle agglutination, PA), MP-IgM (immune colloidal gold technique, GICT), MP-DNA, MP-RNA (simultaneous amplification and testing, SAT) and MP-DNA (real-time polymerase chain reaction, RT-PCR).

Results:
--Among these 830 children, RT-PCR showed that the positive rate was 36.6%, in which the positive rate of macrolide resistance (A2063G mutation) accounted for 86.2% of cases (262/304).

--Using RT-PCR as the standard, MP-RNA (SAT) had the highest specificity (97.5%), and MP-IgM (PA) had the highest sensitivity (74.0%) and Youden index (53.7%).

--If MP-RNA (SAT) was combined with MP-IgM (PA), its Kappa value (0.602), sensitivity (84.2%), specificity (78.7%) and Youden index (62.9%) were higher than those of single M. pneumoniae detection.

Conclusively, a combination of MP-RNA (SAT) plus MP-IgM (PA) might lead to reliable results as an early diagnostic method for children with clinical manifestations of Mycoplasma pneumoniae pneumonia.

Source: https://bmcpediatr.biomedcentral.com/articles/10.1186/s12887-021-02523-4
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