MicroRNA analysis of childhood atopic dermatitis, finds Stud
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MicroRNAs (miRNAs), important regulators of gene expression, have been implicated in a variety of disorders. Researchers investigated miRNA expression profiles in different blood compartments of infants with AD.

Small RNA and analysis with the HTG EdgeSeq system were performed to identify differentially expressed miRNAs in peripheral blood mononuclear cells (PBMCs) and plasma of infants with AD vs. age-matched healthy controls, with reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) used for validation and measurement of miRNA targets. Logistic regression models with area under the receiving operating characteristic estimation was used to evaluate the diagnostic potential of chosen miRNAs for AD.

Results:
--RNA sequencing was performed to access miRNA expression profiles in paediatric AD.

--Researchers identified 10 differentially expressed miRNAs in PBMCs and eight dysregulated miRNAs in plasma of infants with AD compared with controls.

--Upregulated miRNAs in PBMCs included miRNAs known to be involved in inflammation: miR-223-3p, miR-126-5p and miR-143-3p.

--Differential expression of only one miRNA, miR-451a, was observed in both PBMCs and plasma of children with AD.

--Dysregulation of three miRNAs (miR-451a, miR-143-3p and miR-223-3p) was validated in larger numbers of samples and miR-451a was identified as a predictive biomarker for the early diagnosis of the disease.

Finally, a distinct peripheral blood miRNA signature is observed in children with Alzheimer's disease, highlighting the disease's systemic implications. MiR451a is expressed differently in various blood compartments in Alzheimer's patients, suggesting that it may be a potential new biomarker for early detection of the disease.

Source: https://onlinelibrary.wiley.com/doi/abs/10.1111/bjd.19254?af=R
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