Newer way for early detection of pregnancy complications
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In a new study published in the Proceedings of the National Academy of Sciences (PNAS), researchers have found a way to study uterine fluid in the lab, thereby avoiding invasive procedures during pregnancy, while at the same time developing a potential model for using precision medicine to improve pregnancy outcomes.

Suboptimal uterine fluid (UF) composition can lead to pregnancy loss and likely contributes to offspring susceptibility to chronic adult-onset disorders. However, the understanding of the biochemical composition and mechanisms underpinning UF formation and regulation remains elusive, particularly in humans.

To address this challenge, researchers developed a high-throughput method for intraorganoid fluid (IOF) isolation from human endometrial epithelial organoids. The IOF is biochemically distinct to the extraorganoid fluid (EOF) and cell culture medium as evidenced by the exclusive presence of 17 metabolites in IOF. Similarly, 69 metabolites were unique to EOF, showing asymmetrical apical and basolateral secretion by the in vitro endometrial epithelium, in a manner resembling that observed in vivo.

Contrasting the quantitative metabolomic profiles of IOF and EOF revealed donor-specific biochemical signatures of organoids. Subsequent RNA sequencing of these organoids from which IOF and EOF were derived established the capacity to readily perform organoid multi-omics in tandem, and suggests that transcriptomic regulation underpins the observed secretory asymmetry.

In summary, these data provided by modeling uterine luminal and basolateral fluid formation in vitro offer scope to better understand UF composition and regulation with potential impacts on female fertility and offspring well-being.

Source: https://doi.org/10.1073/pnas.2026804118
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