SARS-CoV-2 Infection Diagnosed by Cell Culture in 7-Week-Old
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A 7-week-old Italian baby boy was diagnosed with SARS-CoV-2 infection before the local outbreak by cell-culture isolation, according to a new case report.

The baby presented after having developed a fever to 38 C and vomiting at home. At hospital admission, his temperature was 37.5 C and he had mild leukopenia, pharyngeal hyperemia, and upper respiratory tract inflammation, but no signs or symptoms of pneumonia.

There was no suspicion of SARS-CoV-2 infection, as he had no specific risk factors and the virus had not been reported yet in Parma province. The baby was discharged three days later in good condition and remained healthy in the following weeks.

A nasopharyngeal aspirate, a nasal swab, and a pharyngeal swab were sent to the microbiology and virology labs, where cytopathic effects were detected in cell cultures at day 10. Electron microscopy detected viral particles belonging to the Coronaviridae family, and a subsequent real-time RT-PCR assay confirmed the presence of SARS-CoV-2 RNA.

Subsequently, SARS-CoV-2 RNA was also identified in the stored nasopharyngeal aspirate. Pharyngeal swabs conducted two weeks after discharge were negative for SARS-CoV-2 RNA.

It was quite strange that the parents did not appear to become infected, as they were healthy.

"The natural history of the infection is still unknown, and this should stimulate (us) to collect biological samples of all cases of respiratory infections in babies and adults in order to allow the virologists to apply all the diagnostic tools to detect and then study the virus, (rather than) to stop at the first step of the laboratory diagnosis (usually only RNA detection)," researchers said.

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Dr. S●●●●●m S●●●●y P●●●●●l
Dr. S●●●●●m S●●●●y P●●●●●l General Medicine
This is extremely grave news. No stone should be left unturned until the contact tracing and route of transmission has been confirmed. This disease is truly a man made nightmare.
May 26, 2020Like1