Study finds, Impact of Contact Lens Materials and Cleaning P
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The present study aimed to compare the existing soft contact lens (CL) materials regarding their influence on bacterial biofilm formation and adhesion susceptibility. Then, the study was designed to investigate the effectiveness of various disinfecting solutions and evaluate the ability of cleaning regimens in terms of anti-bacterial adhesion and biofilm removal on different soft CL materials.

The biomass test has examined bacterial biofilm development on CLs. At intervals of 24 hours, adhesion test and conventional platform counting were carried out. A number of CL disinfectant techniques for removing biofilm and reducing bacterial adhesion were evaluated for their efficiency. For morphological evaluation of bacterial biofilm, a Scanning Electron Microscopy (SEM) was conducted.

--Printed hydroxyethyl methacrylate (HEMA) CLs significantly demonstrated more biofilm staining and bacterial attachment when compared with non-printed HEMA CLs, while the Filcon II 3 and Nesofilcon A CLs possessed less biofilm biomass and adherent cells.

--Staphylococcus aureus and Pseudomonas aeruginosa represented the highest biofilm producing bacteria on HEMA-based materials in this study.

--The disinfecting regimen with the highest efficacy was the two-step system, first using multipurpose disinfecting solution containing edetate disodium and sorbic acid (MPDS+EDSA), followed by soaking in multipurpose solution (MPS).

--The regimen demonstrated the greatest effect against the pre-formed biofilm and the adhesion activity of S. aureus and P. aeruginosa on the soft CLs.

--The SEM micrographs confirmed the morphological changes of bacterial biofilm after disinfecting and revealed that the two-step system treated CLs displayed less adherent bacteria.

In conclusion, soft CLs based on HEMA can improve the development and adherence capacity of the bacterial biofilm. The two-step system was the most successful technique for the removal of biofilms in which it is possible to use pre-formed biofilm for more than 6 hours in the disinfecting solution of the non-rubber system.