TMT-based proteomic and bioinformatic analyses of human gran
Now open: Certificate Course in Management of Covid-19 by Govt. Of Gujarat and PlexusMDKnow more...Now open: Certificate Course in Management of Covid-19 by Govt. Of Gujarat and PlexusMDKnow more...
Increasing evidence supports a relationship between obesity and either infertility or subfertility in women. In granulosa cells (GCs) obtained from obese individuals, their mitochondria were damaged and the endoplasmic reticulum stress response was accompanied by dysregulated hormonal synthesis.

As granulosa cells (GCs) are essential for oocyte development and fertility, researchers determined here if the protein expression profiles in the GCs from obese subjects are different than those in their normal-weight counterparts.

GC samples were collected from obese female subjects and normal-weight female subjects who were infertile and underwent IVF treatment due to tubal pathology. A quantitative approach including tandem mass tag labeling and liquid chromatography-tandem mass spectrometry (TMT) was employed to identify differentially expressed proteins. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were then conducted to interrogate the functions and pathways of identified proteins. Clinical, hormonal, and biochemical parameters were also analyzed in both groups.

A total of 228 differentially expressed proteins were noted, including 138 that were upregulated whereas 90 others were downregulated.
--Significant pathways and GO terms associated with protein expression changes were also identified, especially within the mitochondrial electron transport chain.

--The levels of free fatty acids in both the serum and follicular fluid of obese subjects were significantly higher than those in matched normal-weight subjects.

In conclusion, in GCs obtained from obese subjects, their mitochondria were damaged and the endoplasmic reticulum stress response was accompanied by dysregulated hormonal synthesis whereas none of these changes occurred in normal-weight subjects. These alterations may be related to the high FFA and TG levels detected in human follicular fluid.

Reproductive Biology and Endocrinology
Source: https://doi.org/10.1186/s12958-021-00760-x
Like
Comment
Share