The transcriptome characteritics of vestibular organs from d
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This study states that delayed endolymphatic hydrops (DEH) and Menieres disease are different manifestations of the same disease, with dissimilar clinical symptoms. RNA?Seq is a useful to characterize the vestibular pathology based on its transcriptome.

The objective of this study was to identify genes that are related to delayed endolymphatic hydrops (DEH) in patients by RNA?Seq analysis.

Investigators collected the entire vestibular system from four patients with DEH who underwent labyrinthectomy. Three control samples were collected from patients with acoustic neuroma or facial neuroma treated via the translabyrinthine approach. High?throughput RNA?Seq analysis was performed to investigate gene expression in the pathological vestibular system.

The findings suggest;

--The altered gene expression profile suggested that DEH is closely related to neuropathy and autoimmune disease.

--In addition, many of the differentially regulated genes were involved in cell adhesion, suggesting a role of cell adhesion in DEH.

--Immunofluorescence analysis confirmed the expression of PMP2 and CLDN19 in the cytoplasm of hair cells and scattered expression of MPZ at cell junctions.

--The protein expression levels were higher in specimens from patients with Meniere’s disease and DEH compared to controls.

In conclusion, the protein expression profile of vestibular organs in patients with endolymphatic hydrops exhibited a degree of similarity to that of Meniere's disease. Endolymphatic hydrops is characterized by autoimmune abnormalities. DEH and Meniere's disease are likely to be different manifestations of the same disease, with disparate clinical symptoms. RNA?Seq is a useful analytical tool to characterize the vestibular pathology based on its transcriptome.

Clinical Otolaryngology
Source: https://doi.org/10.1111/coa.13748
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