Cytotoxicity of NeoMTA Plus, ProRoot MTA and Biodentine on h
Dental pulp stem cells (DPSCs) play a crucial role in the tissue healing process through odontoblast-like cell differentiation.

The aim of this study was to evaluate the biocompatibility and compare the potential in-vitro cytotoxic effects of NeoMTA Plus, ProRootMTA, and Biodentine on human dental pulp stem cells (hDPSCs).

To assess the effects of NeoMTA Plus, ProRoot MTA, and Biodentine extracts at 1st, 3rd, and 7th d on hDPCs, cell populations were determined by flow cytometry using an Annexin V detection kit. The data were analyzed statistically using the Kruskal–Wallis test.

All groups showed cell viability similar to that of the control group on 1st, 3rd, and 7th d. Although Biodentine exhibited higher cell viability rates than the other material groups, no statistically significant differences were noted between the sampled days.

All materials extracts are not cytotoxic and do not induce apoptosis in the hDPSCs. These results suggest that all the tested materials can lead to positive outcomes when used as reparative biomaterials.

Journal of Dental Sciences