First images of Sars-Cov-2 captured by Indian scientists
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The first images of the coronavirus causing the Covid-19 disease in India have been caught by scientists in Pune, using transmission electron microscope imaging. They were published in the Indian Journal of Medical Research.

The images of Sars-Cov-2, are using the throat swab of the first laboratory-confirmed case in India on January 30, 2020. The woman, among three students studying medicine in Wuhan, was diagnosed with Covid-19 after returning home.

The images of the virus by the Covid-19 cases, Kerala show that the Sars-Cov-2 virus closely resembles the Mers-Cov virus that causes Middle East Respiratory Syndrome coronavirus in 2012, and the 2002 Sars-CoV virus that causes Severe Acute Respiratory Syndrome (SARS) coronavirus.

Gene sequencing of the samples of Kerala done at NIV Pune found the virus was a 99.98% match with the virus in China’s Wuhan.


-- To get the image, his group used centrifugation to remove the debris, a 500 μl sample by the throat swab of the Kerala Covid-19 case confirmed using the highly sensitive, by a real-time, polymerase chain reaction.
-- The supernatant (clear liquid) was removed, filed at a final concentration of 1% glutaraldehyde, and adsorbed on to a carbon-coated 200 mesh copper grid. Negative staining was done with sodium phosphotungstic acid, according to the study.
-- The grid was then examined under 100kV accelerating voltage in a transmission electron microscope and the image was captured using a low-dose mode by a side-mounted camera.

“Seven negative-stained virus particles that looked like coronavirus-like particles were imaged in the fields scanned. These included the round shape of the virus with an average size of 70-80 nm and a cobbled surface structure having envelope projections that averaged 15±2 nm in size,” said Dr. Basu.

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